COMPARATIVE EVALUATION OF ANTIBODY TITER AGAINST PASTEURELLA MULTOCIDA IN RABBITS BY USING FOUR DIFFERENT ADJUVANTS IN HS+BQ COMBO VACCINES

Hemorrhagic Septicemia (HS) caused by Pasteurella multocida is an economically significant disease of bovines that instigates high monetary losses due to unexpected mortality animals in emerging countries like Pakistan. Similarly Black Quarter (BQ) Clostridium chauvoei also acute infectious ailment cattle and buffaloes which severe inflammation ofskeletal cardiac muscles, systemic toxicity a occurs. The only way prevent both diseases effective long lasting vaccination programme. Four HS+BQ combo vaccines adjuvanted with four different adjuvants were prepared. Two prepared addition two oil i.e Montanide ISA-50V-2 Eolane-170 while one vaccine was gel fourth alum. bacterial dry weight P. adjusted 2 mg/0.75 mL the for Cl. set at 2.5 mL. dose all 3 /animal. groups rabbits having five each group selected Veterinary Research Institute, Lahore. From inoculated based containing ISA-50 V2 as adjuvant other adjuvant. Third vaccinated rabbit vaccin All rate 1mL / rabbit. Rabbits again same booster after 27 days. Indirect HemAgglutination test conducted 141 days intervals on serum samples groups. results showed have higher antibody titers compared alum against HS disease.